Biotechnology Laboratory Methods
in the Regulated Work Place
Lisa Seidman
Madison Area Technical College
Funded in part by NSF ATE grants
DUE 9752027 and 0101093
Background for this laboratory manual is provided in “Basic Laboratory Methods for Biotechnology” by Lisa A. Seidman, Madison Area Technical College, and Cynthia J. Moore, Illinois State University, ISBN: 0-13-795535-9, Publisher: Prentice Hall, Copyright: 2000, Format: Spiral Bound; 751 pp.
The printable version of this document.
Table Of Contents
Introductory Notes
Overview
Laboratory Safety
General Laboratory Practices
Working in Groups
SOP Outline
Unit I: Introduction to Laboratory Documentation
Unit I: Introduction to Laboratory Documentation
Laboratory Exercise 1
Keeping a Laboratory Notebook
Classroom Activity 1
Writing and Following an SOP
Unit II: Metrology
Unit II: Metrology
Classroom Activity 2
Constructing a Simple Balance
Laboratory Exercise 2
Measuring Weights; Performance Verification of a Balance
Laboratory Exercise 3
Measuring Volumes; Performance Verification of a Micropipettor
Laboratory Exercise 4
The Creation of Aerosols when Working with Liquids
Laboratory Exercise 5
Measuring pH Accurately
Unit III: Making Solutions
Unit III: Making Solutions
Laboratory Exercise 6
Using Conductivity for Quality Control of Solutions
Laboratory Exercise 7
Working with Tris Buffer
Laboratory Exercise 8
More Practice Making Solutions
Laboratory Exercise 9
Putting it all Together - Making a Product According to the Requirements
of GMP
Unit IV: Spectrophotometry
Unit IV: Spectrophotometry
Laboratory Exercise 10
Absorption Spectra of Colored Dyes
Laboratory Exercise 11
Concentration, Absorbance, and Transmittance
Laboratory Exercise 12
Standard Curves with Food Coloring
Laboratory Exercise 13
A Quantitative Protein Assay
Laboratory Exercise 14
The Diphenylamine Colorimetric Assay and Method Validation
Simulation
Contract Testing Laboratory and Biopharmaceutical Company
Laboratory Exercise 15
UV Spectrophotometry of DNA, RNA, and Proteins
Laboratory Exercise 16
Using Absorbance Spectra for Quality Control
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