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Basic Laboratory
Methods Unit 4:
Spectrophotometry


Power Point Lecture:

Measurement of Light
The Measurement of Light

Laboratory Exercises:

Absorption Spectra
Absorption Spectra of Colored Dyes

C A & T
Concentration, Absorbance, and Transmittance

Standard Curves
Standard Curves With Food Coloring

Protein Assay
A Quantitative Protein Assay

Diphenylamine
The Diphenylamine Assay for DNA and Method Validation
With Simulation: Contract Testing Laboratory and Biopharmaceutical Company

UV
UV Spectrophotometry of DNA, RNA and Proteins

Quality Control
Using Spectrophotometry for Quality Control

 

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Contact Us:

Lisa Seidman
lseidman@matcmadison.edu
(608) 246-6204

Jeanette Mowery
jmowery@matcmadison.edu
(608) 243-4307