Chapter 1: Introduction
Chapter 2: Protein Structure
Chapter 3: Assays and Calculations
Bradford Microassay
Sample: Bradford Assay
ß-Galactosidase Assay
Sample: ß-Galactosidase
Sample: Specific Activity
Sample: Purification Factor
Sample: Yield
Sample: Percent Yield
Chapter 4: Purification Methods
Making the Crude Extract
Ammonium Sulfate Precipitation
Dialysis: Desalting and Buffer Exchange
Ion Exchange Chromatography
Chapter 5: Analytical and Verification
Concentration of Samples
Polyacrylamide Gel Electrophoresis
Staining the Gel
Western Blotting
Chapter 6: Reports
Appendix A: Solutions
Appendix B: Materials and Order Information
Appendix C: Resources
